bacillus subtilis gb03

2021/05/21

Feng, Yue; Liu, Song; Jiao, Yun; Gao, Hui; Wang, Miao; Du, Guocheng; Chen, Jian. Moreover, column transport studies showed higher bacterial retention onto sand grains after exposure to FT. The synthesis of lipopeptides was studied by UV-MALDI TOF. A total of nine gerJ mutants have now been isolated in Bacillus subtilis. Shoot dry weight was increased by 4.0-fold (P < 0.05) under non-saline conditions; compared to corresponding media control, GB03 significantly improved shoot dry weight by 4.9-fold (P < 0.05), 6.4-fold (P < 0.01), and 3.2-fold (P < 0.05) with 50, 100, and 150 mM NaCl treatment, respectively (Figure (Figure2B).2B). Because of the ease of protein recovery and availability of protease-deficient strains, the Bacillus subtilis expression-secretion system is an attractive system for streptavidin production. Because molecular studies in this bacterium can be challenging, we aimed at exploiting the genetically highly tractable Gram-positive model organism Bacillus subtilis as a heterologous host. Growth at pH 9 upregulated arginine catabolism (roc), which generates organic acids, glutamate synthase (gltAB), polyamine acetylation and transport (blt), the K(+)/H(+) antiporter (yhaTU), and cytochrome oxidoreductases (cyd, ctaACE, and qcrC). Finally, motility assays revealed that 0.01 and 1g/ml AgNPs, respectively, promoted surface movement in E. coli and B. subtilis under aerobic and anaerobic conditions. Efficient adaptation mechanisms are necessary to cope with this wide range of environmental challenges.

Hu, Junlang; Lei, Pan; Mohsin, Ali; Liu, Xiaoyun; Huang, Mingzhi; Li, Liang; Hu, Jianhua; Hang, Haifeng; Zhuang, Yingping; Guo, Meijin, Riboflavin, an intermediate of primary metabolism, is one kind of important food additive with high economic value. Draft genome of bagasse-degrading bacteria Bacillus aryabhattai GZ03 from deep sea water.

Due to its pH profile and optimum, it could be an interesting candidate for feed applications. Microorganisms of specially Bacillus genera serve as a vast repository of diverse set of industrially important enzymes and utilized for the large-scale enzyme production using a fermentation technology. One group of strains had spores with an average D120 C of 0.33 s, while the spores of the other group displayed significantly higher heat resistances, with an average D120 C of 45.7 s. When comparing spore inactivation data obtained using batch- and continuous flow heating, the z-values were significantly different, hence extrapolation from one system to the other was not justified. The samples where irradiated during the exponential growth phase for 1, 2, and 24 hours. Loss of aerial hyphae in developmental mutants can be rescued by addition of purified SapB.

In Bacillus subtilis, the termination of DNA replication via polar fork arrest is effected by a specific protein:DNA complex formed between the replication terminator protein (RTP) and DNA terminator sites. GB03 increased plant biomass of white clover under both non-saline conditions and salinity stress (Figure (Figure2).2).

By using this strain, 10g/l of MI initially included in the medium was completely converted into SI within 48h of cultivation in a rich medium containing 2% (w/v) Bacto soytone. Cost-effective medium for the production of mosquito pupicidal lipopeptide from Bacillus subtilis subsp. However, an additional CcpA-independent mechanism represses pftAB in the presence of malate. Proteases are the most important group of enzymes utilized commercially in various arenas of industries, such as food, detergent, leather, dairy, pharmaceutical, diagnostics, and waste management, accounting for nearly 20% of the world enzyme market.

A Canonical Biotin Synthesis Enzyme, 8-Amino-7-Oxononanoate Synthase (BioF), Utilizes Different Acyl Chain Donors in Bacillus subtilis and Escherichia coli.

Here we show that the exposure of B. subtilis to surfactin at concentrations of 350 and 650 g/ml (designated as SF350 and SF650, respectively) leads to a concentration-dependent growth arrest followed by regrowth with an altered growth rate. Strains can be separated into two groups, to which different spore heat inactivation kinetics apply. We propose that the assembly of the previously unreported fibrous layer, which we link to the darkly stained outer coat seen by electron microscopy, and the nanodot layer are cotH- and cotE- dependent and cotE-specific respectively. Kim, S-Y; Jo, E-K; Kim, H-J; Bai, K; Park, J-K. To investigate the microbicidal mechanisms of high-power microwave (2.0 kW) irradiation on Bacillus subtilis and to determine the effect of this procedure on the ultrastructure of the cell wall.

stephensi (2.3 g/ml) was comparable to that obtained with CMM from the conventional medium. Knowledge of the highly regulated processes governing the production of flagella in Bacillus subtilis is the result of several observations obtained from growing this microorganism in liquid cultures.

A strain probably carrying a lacZ fusion to the gerJ promoter demonstrated increased expression between t2 and t4. Based on the spore heat resistance data, two distinct groups (p < 0.001) within the B. subtilis group could be identified. These two factors can be engineered to reduce the electrostatic interaction between each other during the secretion process, which finally leads to a better secretion yield of -amylases. Bacillus subtilis biofilms contain TasA as major proteinaceous component in addition to exopolysaccharides. The phylogenetic tree analysis results showed that the isolate was 99% related to B. subtilis BSF01. Here, we revealed that the soil bacterium Bacillus amyloliquefaciens A50 is tolerant to the non-canonical amino acid D-tyrosine (D-Tyr), in contrast to the closely related Bacillus strain B. subtilis 168, which is a widely used "domesticated" laboratory strain.

Rhizosphere microbes as essential partners for plant stress tolerance.

In response to low DO tension in broth, the glucose metabolic flux through Embden-Meyerhof-Parnas (EMP) pathway was higher and the gene, alsS, encoding for acetolactate synthase was significantly activated that may result due to lower ATP concentration and higher NADH/NAD + ratio. However, this compound is relatively rare, whereas another stereoisomer of inositol, myo-inositol (MI) is abundant in nature.

Moreover, challenge with specific strains overproducing surfactin and mycosubtilin led to a slightly enhanced stimulation of the defence response compared with the LP-non-producing strain of B.subtilis.

The KKU213 strain produced extracellular amylase, cellulase, lipase and protease, is highly acid-resistant (pH 2) when cultured in inulin and promotes health and reduces infection of intestinally colonized broiler chickens. In B. anthracis and Bacillus subtilis htrC mutants, YpeB was partially stabilized during germination but was still degraded at a reduced rate by other, unidentified proteases. In addition, self-sensing in the ComQXP system affected persistence to antibiotic treatment.

In the non-treated control group, 13.1% mortality was observed versus 7.8% in the probiotic-treated group (P = 0.33), with mortality being mainly due to diarrhoea. The average daily milk yield per ewe was significantly lower in the control group (0.80 l) than that in the probiotic-treated group (0.93 l) (P < 0.05). From these results, it was determined that the two bacilli significantly inhibited the growth of an important phytopathogenic fungus by at least two different mechanisms: lipopeptide synthesis and competition among microorganisms. Then, when the glucose concentration was increased to 100g/L, the acetoin yield reached 24.6g/L, but 2.4g/L of 2,3-butanediol was detected at the end of fermentation.

On the basis of the nucleotide sequence of a conserved segment of the 16S rRNA gene, the bacterium has been identified as B. subtilis DJM-51. Geosmin and 2-methylisoborneol (2-MIB) are two of the most common taint compounds that adversely affect the quality of aquacultural animals. Decolourization of 4-Chloro-2-Nitrophenol by a Soil Bacterium.

These quantum dots were solubilized with mercaptoacetic acid and conjugated to adenine. We further propose that the inner coat multilayer structure is crystalline with its apparent two-dimensional (2D) nuclei being the first example of a non-mineral 2D nucleation crystallization. The growth promotion of white clover by GB03 inoculation in soil evaluated in the present study is consistent with previous reports in Arabidopsis (Zhang et al., 2007, 2008a,b, 2010b; Xie et al., 2009; Par et al., 2011). Statistical analyses, One-Way ANOVA and Duncan's multiple range tests, were performed. Swarming differentiation and swimming motility in Bacillus subtilis are controlled by swrA, a newly identified dicistronic operon. The BioF acyl chain donor has long been thought to be pimeloyl-CoA. The bacterial cell wall is essential for viability and shape determination.

The results indicated that the transcription levels of four virulence genes of A. hydrophila, including aer, ahyB, hcp, and emp, decreased when A. hydrophila was cultured with B. subtilis CH9. The results demonstrate that E. coli and B. subtilis react differently from AgNPs over a wide range of sublethal concentrations examined under both aerobic and anaerobic conditions. Cao, Haojie; van Heel, Auke J; Ahmed, Hifza; Mols, Maarten; Kuipers, Oscar P. Bacillus subtilis is widely used as a cell factory for numerous heterologous proteins of commercial value and medical interest. (2006). These authors have contributed equally to this work.

Presence of LacA protein is verified by western blotting. The results confirmed the Ag species in Ag NP-treated B. subtilis cells as Ag2O, indicating that Ag-NP toxicity is likely mediated by released Ag+ ions from Ag NPs, which penetrate bacterial cells and are subsequently oxidized intracellularly to Ag2O. Berendsen, Erwin M; Zwietering, Marcel H; Kuipers, Oscar P; Wells-Bennik, Marjon H J. By evaluating the absorption of each well at 600nm immediately before and after irradiation, the population density within each well was assessed. We determine directly for the first time that Cu adsorbs to B. subtilis as a (CuO 5H n) n-8 monodentate, inner-sphere surface complex involving carboxyl surface functional groups. Our results show strong, compartment-specific expression of IpHluorin that allowed accurate pHi measurements of live cultures during exponential growth, early and late sporulation, spore germination, and during subsequent spore outgrowth. Screening a genome-wide transposon mutant library, we find that an active malic enzyme replenishing the pyruvate pool is required for this repression. 31172256, 31222053), the Programs for New Century Excellent Talents, Ministry of Education, China (grant No. A time resolved metabolomics study: the influence of different carbon sources during growth and starvation of Bacillus subtilis.

(2006). These findings provide conclusive evidence for the role of Ag+ ions in Ag-NP microbial toxicity, and suggest that the impact of inappropriately disposed Ag NPs to soil and water ecosystems may warrant further investigation. Analysis of the membrane lipid composition revealed modifications both in the polar head group and the fatty acid region. The production of highly efficient, recyclable and cost-effective enzymes is one of the most important goals in industrial biotechnology. Construction of a highly efficient Bacillus subtilis 168 whole-cell biocatalyst and its application in the production of L-ornithine. Conversion of protein wastes into biofuels and ammonia by engineering nitrogen flux in Escherichia coli has been demonstrated as a method to reclaim reduced-nitrogen and curb its environmental deposition. Received 2014 Aug 19; Accepted 2014 Sep 16.

This paper defines an enzyme of the Bacillus subtilis pathway and shows that it differs from that of Escherichia coli in the ability to utilize specific precursors.

Farag M. A., Ryu C. M., Sumner L. W., Par P. W. (2006). Inhibitory activities of partially purified bacteriocin on all the DFU isolates were done by agar well diffusion method. Collectively, our data suggest that condensin resolves replicated origins by promoting the juxtaposition of DNA flanking parS sites, drawing sister origins in on themselves and away from each other. It was found from macroscopic observations that the characteristic quantities for the periodic growth such as the migration time, the consolidation time and the terrace spacing do not depend so much on nutrient concentration Cn, but do on agar concentration Ca. Surfactin, an anionic lipopeptide produced by Bacillus subtilis, is an antimicrobial that targets the cytoplasmic membrane. rhizome ethanol extract against MRSA, MRCNS, MSSA, Analysis of the Function of a Putative 2,3-Diphosphoglyceric Acid-Dependent Phosphoglycerate Mutase from, Mucosal immune response in broilers following vaccination with inactivated influenza and recombinant, Expression and characterization of aiiA gene from, Differences in the roles of a glutamine amidotransferase subunit of pyridoxal 5'-phosphate synthase between, Galactose metabolism plays a crucial role in biofilm formation by, Mechanisms of adaptation to nitrosative stress in, Functional Diversity of AAA+ Protease Complexes in, A part toolbox to tune genetic expression in, The comER Gene Plays an Important Role in Biofilm Formation and Sporulation in both, Heterologous expression of antigenic peptides in, Analysis of Host-Takeover During SPO1 Infection of, Small regulatory RNA-induced growth rate heterogeneity of, Transcriptional Profiling in Cotton Associated with, Simple, Inexpensive, and Rapid Way to Produce, Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on, DNA gyrase inhibitors block development of, A Combinatorial Kin Discrimination System in, Dimethylglycine Provides Salt and Temperature Stress Protection to, Properties of Thermosensitive Extracellular -Amylases of, Ultrasensitive electrochemical biosensor for detection of DNA from, Oscillating behavior of Clostridium difficile Min proteins in, Folic acid and the methylation of homocysteine by, Enhanced production of recombinant nattokinase in, Antimicrobial and plant growth-promoting properties of the cacao endophyte, Purification, Characterization and Comparison between Two New L-asparaginases from, Purine biosynthesis is the bottleneck in trimethoprim-treated, First steps of bacteriophage SPP1 entry into, Inhibitory activity of surfactin, produced by different, Strategy to approach stable production of recombinant nattokinase in, MotPS is the stator-force generator for motility of alkaliphilic, Homogeneity and heterogeneity in amylase production by, Thermal Regulation of Membrane Lipid Fluidity by a Two-Component System in ", Purification and characterization of nattokinase from, Design of a CRISPR-Cas system to increase resistance of, Bacterial determinants of the social behavior of, The immunological characteristics and probiotic function of recombinant, Cortex content of asporogenous mutants of, A new maltose-inducible high-performance heterologous expression system in, Metabolic pathway analysis and kinetic studies for production of nattokinase in, Comparison of plant growth-promotion with Pseudomonas aeruginosa and, Partial biochemical characterization of crude extract extracellular chitinase enzyme from, Comparative evaluation of extracellular -D-fructofuranosidase in submerged and solid-state fermentation produced by newly identified, Cloning and characterization of the metE gene encoding S-adenosylmethionine synthetase from, Fungal Competitors Affect Production of Antimicrobial Lipopeptides in, Inhibition of quorum sensing-mediated virulence in Serratia marcescens by, Effects of microbial loading and sporulation temperature on atmospheric plasma inactivation of, Sporulation during growth in a gut isolate of, Sporulation during Growth in a Gut Isolate of, Foam Separation of Pseudomonas fluorescens and, Foam separation of Pseudomonas fluorescens and, Fitness Trade-Offs in Competence Differentiation of, Hematite enhances the removal of Cr(VI) by, Extracellular Vesicles Produced by the Gram-positive Bacterium, Translation Control of Swarming Proficiency in, Isolation and Characterization of Phages Infecting, Control of Initiation of DNA Replication in, Production of nattokinase by batch and fed-batch culture of.

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